ABSTRACT
Meningitis is a common evolution in progressive disseminated histoplasmosis in children, and is asymptomatic in many cases. In leukemia, the impaired of the T cells function can predispose to the disseminated form. The attributed mortality rate in this case is 20 percent-40 percent and the relapse rate is as high as 50 percent; therefore, prolonged treatment may be emphasized. We have described a child with acute myeloid leukemia (AML), that developed skin lesions and asymptomatic chronic meningitis, with a good evolution after prolonged treatment with amphotericin B deoxycholate followed by fluconazole.
Subject(s)
Adolescent , Humans , Male , Histoplasmosis/diagnosis , Leukemia, Myeloid/immunology , Meningitis, Fungal/diagnosis , Acute Disease , Amphotericin B/therapeutic use , Antifungal Agents/therapeutic use , Chronic Disease , Drug Combinations , Drug Therapy, Combination , Deoxycholic Acid/therapeutic use , Fluconazole/therapeutic use , Histoplasmosis/drug therapy , Immunocompromised Host , Leukemia, Myeloid/microbiology , Meningitis, Fungal/drug therapy , Meningitis, Fungal/microbiology , Treatment OutcomeABSTRACT
Samples of Schistosoma mansoni soluble adult worm proteins (SWAP) were iodinated with 4-15 µmolI/mg protein using iodine monochloride. The capacity to elicit immediate hypersensitivity reactions of the iodinated derivatives was compared to that of the native SWAP preparations. The degranulation of mast clls from infected mice decreased with increasing iodine incorporation and was absent in fully iodinated samples containing 15 µmol I/mg protein. The response of guinea pigs and humans to the intradermal test with iodinated SWAP also decrease in proportion to iodine incorporation, and no responses were obtained with fully iodinated samples. No false-positive tests were observed. Antibodies to the folly iodinated extracts generated in C57BL/10 mice reacted by ELISA with the unmodified proteins and by immunoprecipitation on agar gel. The immunoprecicpitation pattern suggested that some epitopes were altered by iodination
Subject(s)
Antibodies, Helminth/biosynthesis , Hypersensitivity, Immediate , Helminth Proteins/pharmacology , Schistosoma mansoni/immunology , Chagas Disease/immunology , Chlorides , Enzyme-Linked Immunosorbent Assay , Iodides , Precipitin TestsABSTRACT
Os soros de 472 brasileiros, confirmados como sendo positivos ou negativos em relacao a presenca de anticorpos anti-HIV e compreendendo todo o espectro clinico da infeccao, foram utilizados na avaliaco de seis ensaios imunoenzimaticos comerciais (ELISA), bem como de quatro testes alternativos tais como imunofluorescencia indireta (IFI), hemaglutinacao passiva (HP), dot blot e Karpas AIDS cell test. As sensibilidades variaram de 100 por cento (ELISA Abbott e Roche) a 84,2 por cento (HP) e as especificidades variaram de 99,3 por cento (IFI) a 80,2 por cento (HP). A sensibilidade e especificidade da HP e a sensibilidade do Karpas AIDS cell test foram significativamente menores que os outros ensaios. Embora a IFI e o dot blot tivessem apresentado uma boa sensibilidade e especificidade, os ensaios imunoenzimaticos (ELISA) foram mais adequados para serem utilizados em triagem quando outros parametros tais como facilidade de leitura e interpretacao dos resultados e duracao dos ensaios foram considerados